| Congresso Brasileiro de Microbiologia 2023 | Resumo: 194-1 | ||||
Resumo:In Brazil, the genus Staphylococcus spp. is considered as the main causal agent of bovine mastitis. Several studies indicate that both S. aureus bacteria and coagulase-negative staphylococci, in addition to being the most frequent agents found in mastitis, they have an increasing pattern on resistance to antimicrobials. There are several alternatives aiming to reduce costs and, mainly, to minimize the processes of microbiological resistance. One of those alternatives is the use of phytotherapeutic agents (or derivatives thereof) as therapeutic adjuvant complements. Jambú (Spilanthes acmella) is one of cases, since it is a plant rich in bioactive isobutyl amides. Due to the presence of this substance, the plant has traditional applications in pharmaceutical products, food, and products for health and personal care. Thus, the objective of this study was to evaluate the antibacterial effects of the Jambú (crude plant extract) in isolates of Staphylococcus spp. obtained from bovine milk samples. For this purpose, 60 milk samples were collected in order to obtain colonies Staphylococcus spp. for further antibacterial tests using crude plant extract of S. acmella. As a result, from the 60 milk samples, it was possible to obtain characteristic colonies of Staphylococcus spp. in 56 (93,3%) samples. From these, 19 (33,9%) were characterized as Staphylococcus aureus and 37 (66,1%) as Staphylococcus spp. All 56 strains showed some level of sensitivity to the Jambú, from the initial dilution (20% concentration) to the final dilution (0,039%). In our study, we obtained a high percentage of isolation (93,3%) of Staphylococcus spp. from the samples, with 33,9% representing Staphylococcus aureus and 66,1% of coagulase-negative Staphylococcus. In this scenario, we found that the Jambú crude plant extract inhibited the growth of Staphylococcus spp. isolated from bovine milk samples, in all tested concentrations, except the last one (at 0,0195%). The isolates that exhibited lower inhibitory concentration, the effects, for the most part, behaved as bacteriostatic, whereas for the isolates with a higher inhibitory concentration the effect behaved as bactericide. We believe that these differences on effects of concentration may be linked to different levels of sensitivity of the field isolates. Palavras-chave: microbiology, dairy cattle, growth inhibition Agência de fomento:Fundação de Amparo à Pesquisa do Estado do Amazonas | |||||